Lytic effect of Bacillus subtilis elastase on gram-positive and negative bacteria.
Indian J Exp Biol
;
1992 May; 30(5): 380-3
Artigo
em Inglês
| IMSEAR
| ID: sea-58536
ABSTRACT
Elastase of B. subtilis 6a caused lysis of freshly grown cells of Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, Salmonella typhi and Pseudomonas aeruginosa and Gram-positive (B. subtilis) bacteria. Heat killed and lyophilised Gram-positive and negative bacteria showed higher sensitivity to elastase. Both Gram-negative and Gram-positive bacteria were lysed maximally by elastase at pH 8.0. At this pH, activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
Texto completo:
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Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Bacillus subtilis
/
Proteínas de Bactérias
/
Bacteriólise
/
Elastase Pancreática
/
Bactérias Gram-Negativas
/
Bactérias Gram-Positivas
/
Concentração de Íons de Hidrogênio
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1992
Tipo de documento:
Artigo
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