Reversible male sterility in transgenic tobacco carrying a dominant-negative mutated glutamine synthetase gene under the control of microspore-specific promoter.
Indian J Exp Biol
;
2007 Dec; 45(12): 1022-30
Artigo
em Inglês
| IMSEAR
| ID: sea-58552
ABSTRACT
Metabolic engineering was used to disrupt glutamine metabolism in microspores in order to block pollen development. We used a dominant-negative mutant (DNM) approach of cytosolic glutamine synthetase (GS1) gene under the microspore-specific promoter NTM19 to block glutamine synthesis in developing pollen grains. We observed partial male sterility in primary transgenic plants by using light microscopy, FDA, DAPI and in vitro pollen germination test. Microspores started to die in the early unicellular microspore stage, pollen viability in all primary transgenic lines ranged from 40-50%. All primary transgenics produced seeds like control plants, hence the inserted gene did not affect the sporophyte and was inherited through the female germline. We regenerated plants by in vitro microspore embryogenesis from 4 individual lines, pollen viability of progeny ranged from 12 to 20%, but some of them also showed 100% male sterility. After foliage spray with glutamine, 100% male-sterile plants were produced viable pollen and seed set was also observed. These results suggested that mutated GS1 activity on microspores had a significant effect on normal pollen development. Back-cross progenies (T2) of DH 100% male-sterile plants showed normal seed set like primary transgenics and control plants.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Pólen
/
Nicotiana
/
Regiões Promotoras Genéticas
/
Plantas Geneticamente Modificadas
/
Infertilidade das Plantas
/
Genes Dominantes
/
Aminoácidos
/
Glutamina
/
Glutamato-Amônia Ligase
/
Mutação
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2007
Tipo de documento:
Artigo
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