Comparison of two colorimetric assays to determine viral infectivity in micro culture virus titration.
Indian J Exp Biol
;
1999 Dec; 37(12): 1223-6
Artigo
em Inglês
| IMSEAR
| ID: sea-58690
ABSTRACT
Efficacy of two colorimetric assays, viz. MTT (3-4,5-dimethylthiazol-2-(yl-2,5-diphenyl tetrazolium bromide) and neutral red (NR) assays, performed by integrating them to micro culture virus titration (MCVT), was compared with the conventional MCVT method in terms of percentages of infectivity and 50% infectivity end points by employing Polio virus type-3 and Dengue virus type 4 as the candidate viruses. The results suggested that MTT assay has an edge over NR assay as well as conventional MCVT method. For the first time, NR assay has been successfully employed for the determination of virus infectivity titre.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Sais de Tetrazólio
/
Tiazóis
/
Virologia
/
Células Vero
/
Humanos
/
Linhagem Celular
/
Chlorocebus aethiops
/
Colorimetria
/
Efeito Citopatogênico Viral
/
Poliovirus
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1999
Tipo de documento:
Artigo
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