Influence of melatonin on blood morphology in male albino mice.

Influence of melatonin on diurnal changes in hematological profiles was examined in male albino mice, at six hourly interval. Melatonin treatment either once daily (25 micrograms at 1700 hrs) or twice daily (25 micrograms at 0900 and 1700 hrs) for two weeks resulted in an alternation in the RBC rhythm. Generally the effect was suppressive though at some times the counts were marginally increased. Total WBC counts were increased and there was apparently a change in their rhythm too. Interestingly the differential WBC counts exhibited different patterns in, once and twice daily melatonin-treated mice. Melatonin given once daily predominantly stimulated the absolute lymphocyte count whereas the twice daily regimen predominantly stimulated the neutrophil count. This perhaps is related to the varied exposures of the animals to melatonin in the two experimental setups. Erythrocyte indices like mean cell volume, mean cell hemolglobin and mean cell hemoglobin concentration in both the experiments correlated with hemoglobin and hematocrit values. It may be concluded that melatonin has a modulatory role in hemopoiesis and its rhythms. The stimulatory effect of melatonin on WBC supports its purported immunopotentiating action.