Catalytic and regulatory properties of sulphur metabolizing enzymes in cyanobacterium Synechococcus elongatus PCC 7942.
Indian J Exp Biol
;
2006 Sep; 44(9): 767-72
Artigo
em Inglês
| IMSEAR
| ID: sea-58940
ABSTRACT
Synechococcus elongatus PCC 7942 was able to grow with several S sources. The sulphur metabolizing enzymes viz. ATP sulphurylase, cysteine synthase, thiosulphate reductase and L- and D-cysteine desulphydrases were regulated by sulphur sources, particularly by sulphur amino acids and organic sulphate esters. Sulphur starvation reduced ATP sulphurylase and cysteine synthase whereas reduced glutathione appreciated Cys degradation activity. With partially purified enzymes apparent Km values for sulphate, ATP, D- and L-Cys, thiosulphate, sulphide and O-acetyl serine were in a range of 12-50 microM. p-Nitrophenyl sulphate inhibited ATP sulphurylase competitively. Met was a feedback inhibitor of several key enzymes.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Sulfato Adenililtransferase
/
Compostos de Enxofre
/
Sulfurtransferases
/
Catálise
/
Cromatografia DEAE-Celulose
/
Cistationina gama-Liase
/
Cisteína Sintase
/
Synechococcus
/
Inibidores Enzimáticos
/
Oxirredutases atuantes sobre Doadores de Grupo Enxofre
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2006
Tipo de documento:
Artigo
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