Construction of chickpea genomic library.
Indian J Exp Biol
;
1989 Oct; 27(10): 858-60
Artigo
em Inglês
| IMSEAR
| ID: sea-58991
ABSTRACT
For construction of chickpea genomic library, DNA was isolated, purified on CsCl gradient and size fractionated into 15-20 Kb fragments after restriction with Sau 3A. These fragments were ligated to phage lambda (EMBL-3) vector and the recombinant molecules packaged in vitro into viable phage particles. The recombinant phages were obtained as phages on a P2 lysogen of E. coli (Spi- selection) and amplified to establish a permanent library. This is the first report of the construction of chickpea genomic library.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plantas Medicinais
/
DNA
/
Biblioteca Genômica
/
Fabaceae
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1989
Tipo de documento:
Artigo
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