Binary conjugational transfer system of vectors pRK290 and pLAFRI is proficient both ways between Escherichia coli and Rhizobium.
Indian J Exp Biol
;
1990 Oct; 28(10): 994-5
Artigo
em Inglês
| IMSEAR
| ID: sea-59151
ABSTRACT
Wide host range vector plasmids pRK290 and pLAFRI carrying genomic fragments of Rhizobium are transferable both ways between R. meliloti and R. leguminosarum cells on the one hand and to E. coli cells on the other, in triparental matings involving E. coli cells carrying pRK2013, the helper for Tra functions to the vector plasmids. The vector plasmids pRK290 and pLAFRI can be employed for recovering clones harbored by R. leguminosarum and R. meliloti by transfer to Rhizobium cells by direct matings of the library with them.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasmídeos
/
Rhizobium
/
Clonagem Molecular
/
Conjugação Genética
/
Escherichia coli
/
Vetores Genéticos
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1990
Tipo de documento:
Artigo
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