Partial purification and cytotoxic effects of Salmonella proteinous moieties on chick embryo fibroblasts.
Indian J Exp Biol
;
2006 Aug; 44(8): 666-70
Artigo
em Inglês
| IMSEAR
| ID: sea-59305
ABSTRACT
Salmonella enterica serovars, viz., S. Weltevreden, S. Typhimurium, S. Gallinarum and S. Bareilly were treated with cephotaxime to release of intracellular proteins. The cephotaxime extract (CE) was salt precipitated with ammonium sulphate (45-70%) and dialyzed, and denoted as precipitated dialyzed proteins (PDP). Further, both CE and PDP of Salmonella Weltevreden and PDP of rest of the serovars were subjected to gel filtration using Sephacryl S-200HR. Different fractions along with CE and PDP were studied for their cytotoxicity using chicken embryo fibroblast (CEF). All the CE and PDP exerted cytotoxic effects, characterized by rounding, detachment, shrinkage and clumping of cells with syncytia formation. Also, the fractions eluted in the 2nd and 3rd peaks through Sephacryl S-200HR column invariably had cytotoxic activity. It was concluded that in place of Vero cell line, CEF cells could also be used to test cytotoxicity.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Salmonella
/
Proteínas de Bactérias
/
Células Cultivadas
/
Embrião de Galinha
/
Cromatografia em Gel
/
Morte Celular
/
Forma Celular
/
Fibroblastos
/
Animais
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2006
Tipo de documento:
Artigo
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