In vitro regeneration of roots of Phyla nodiflora and Leptadenia reticulata, and comparison of roots from cultured and natural plants for secondary metabolites.
Indian J Exp Biol
;
2002 Dec; 40(12): 1382-6
Artigo
em Inglês
| IMSEAR
| ID: sea-59400
ABSTRACT
Adventitious roots, generated using leaf explants of P. nodiflora, and meristem explants of L. reticulata, were cultured on Murashige and Skoog (MS) medium supplemented with napthylacetic acid (2 microM) and indole butyric acid (3 microM) respectively. After 30 days, subculturing of roots in liquid MS medium with napthylacetic acid (1.5 microM) for P. nodiflora and indole butyric acid (3 microM) for L. reticulata afforded considerable increase in root mass. HPTLC profiles and microscopic examination of transverse sections of in vitro and naturally grown roots provided information on secondary metabolite accumulation vis-à-vis developmental stages of the root.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Regeneração
/
Cromatografia em Camada Fina
/
Raízes de Plantas
/
Apocynaceae
/
Verbenaceae
/
Animais
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2002
Tipo de documento:
Artigo
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