Effect of aflatoxin B in vitro on rat liver mitochondrial respiratory functions.
Indian J Exp Biol
;
1997 Nov; 35(11): 1187-90
Artigo
em Inglês
| IMSEAR
| ID: sea-59481
ABSTRACT
Isolated rat liver mitochondria were incubated with various concentrations of aflatoxin B (AFB) for different periods of time. Respiration rates were then measured with two substrates (succinate and glutamate). State 3 respiration rate (with added ADP) declined with increase in preincubation concentrations of AFB1 (0.15-0.50 mM). On the other hand, state 4 respiration rate (after depletion of added ADP) was found to increase with increased pretreatment concentration of AFB. As a consequence, respiratory control index was reduced attaining minimum value with 0.25 mM AFB and preincubation time of 10 min. The induced anomaly in mitochondrial respiratory functions appear to be due to membrane damage caused by interaction of reactive AFB1 metabolite generated by mitochondrial cytochrome P-450 enzymes with mitochondrial components.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Consumo de Oxigênio
/
Ratos
/
Masculino
/
Mitocôndrias Hepáticas
/
Carcinógenos
/
Células Cultivadas
/
Aflatoxina B1
/
Ratos Wistar
/
Animais
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1997
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS