Production of ampicillin through Escherichia coli NCIM 2563 immobilized and cross-linked in alginate beads.
Indian J Exp Biol
;
1994 Sep; 32(9): 672-3
Artigo
em Inglês
| IMSEAR
| ID: sea-59505
ABSTRACT
6-Amino penicillanic acid (6-APA) was condensed with D-alpha-phenyl glycine chloride in presence of E. coli NCIM 2563 to form ampicillin. At pH 5, 30% of 6-APA was converted to ampicillin during 1 hr incubation. When E. coli cells were immobilized in calcium alginate beads, the activity remained unaffected even after six batches of bioconversion. Inclusion of glutaraldehyde as multifunctional cross-linking agent, improved the stability of the biocatalyst beads.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ácido Glucurônico
/
Reagentes de Ligações Cruzadas
/
Alginatos
/
Escherichia coli
/
Ácidos Hexurônicos
/
Ampicilina
/
Microesferas
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1994
Tipo de documento:
Artigo
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