Localization of hup determinants in Rhizobium sp. sesbania.
Indian J Exp Biol
;
1998 Feb; 36(2): 209-12
Artigo
em Inglês
| IMSEAR
| ID: sea-59542
ABSTRACT
Rhizobium sp. sesbania strain SRS 78, a Hup wild type carrying three plasmids was mutagenised using Tn5-sac BR cassette and clones carrying Tn5 insertion in plasmids were identified on the basis of transfer frequency of Tn5-sac BR labelled plasmids from Rhizobium to A. tumefaciens. Neomycin sensitive (Nms) clones were isolated from clones containing Tn5 labelled plasmids by plating on sucrose (5%). Nms clones did not show loss of hup activity. Preliminary results on curing studies indicated that hup genes are located on chromosome. These results were later confirmed by southern hybridization using plasmid pKHT30 containing hup genes of Azotobacter chroococcum. None of the three plasmids showed hybridization with hup gene probe while chromosomal DNA left in the wells showed positive signal.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasmídeos
/
Rhizobium
/
Proteínas de Bactérias
/
Elementos de DNA Transponíveis
/
Mutagênese
/
Proteínas de Ligação a DNA
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1998
Tipo de documento:
Artigo
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