In vitro formation of organ specific proximate carcinogen of benzo(a)pyrene by rat homogenates.
Indian J Exp Biol
;
2002 Sep; 40(9): 1067-70
Artigo
em Inglês
| IMSEAR
| ID: sea-59560
ABSTRACT
In order to determine the organ specific carcinogenicity of benzo(a)pyrene (B(a)P), its metabolites, formed in vitro by incubation with the homogenates from liver, lungs, kidneys, intestine and brain of rats, were isolated by TLC and spectroscopy. B(a)P was found to be converted into a number of metabolites by different tissue homogenates. The results showed that the proximate carcinogenic metabolite, 7,8-dihydro-7,8-dihydroxy B(a)P was formed only when rat lung and kidney homogenates were incubated with B(a)P in vitro. The UV spectral analysis also confirmed the formation of this metabolite only on incubation of B(a)P with rat lung and kidney homogenates. As the proximate carcinogenic metabolite was only formed by incubating B(a)P with the homogenates from target organs, its organ specific carcinogenicity may be explained.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Especificidade de Órgãos
/
Ratos
/
Espectrofotometria Ultravioleta
/
Benzo(a)pireno
/
7,8-Di-Hidro-7,8-Di-Hidroxibenzo(a)pireno 9,10-óxido
/
Encéfalo
/
Carcinógenos
/
Cromatografia em Camada Fina
/
Ratos Wistar
/
Intestinos
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2002
Tipo de documento:
Artigo
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