Detection of px gene product of bovine leukemia virus in infected cells.
Indian J Exp Biol
;
1990 Dec; 28(12): 1107-11
Artigo
em Inglês
| IMSEAR
| ID: sea-59934
ABSTRACT
Bovine leukemia virus (BLV), like its closest relatives human T-cell leukemia virus-I and II, contain a 'px' gene, between the 'env' gene and the 3' long terminal repeat in its genome. A monoclonal antibody prepared against a synthetic oligopeptide whose sequence was deduced from highly conserved region of 'px' gene of BLV, was used to detect the presence of 'px' gene product in chronically BLV infected synchronised cells. By immunoperoxidase staining the 'px' gene product was detected maximum after 6-9 hr after synchronization in the nucleus of the cells which demonstrated the close interaction of it with viral DNA which is integrated with host cell genome.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas Virais
/
Dados de Sequência Molecular
/
Proteínas Oncogênicas de Retroviridae
/
Células Cultivadas
/
Produtos do Gene tax
/
Genes pX
/
Sequência de Aminoácidos
/
Vírus da Leucemia Bovina
/
Animais
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1990
Tipo de documento:
Artigo
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