Your browser doesn't support javascript.
loading
Construction and screening of subgenomic library of chickpea (Cicer arietinum L.) for legumin genes and their analysis.
Indian J Exp Biol ; 1996 Jun; 34(6): 496-501
Artigo em Inglês | IMSEAR | ID: sea-59946
ABSTRACT
Legumin storage protein genes of chickpea were identified by hybridizing restricted chickpea genomic DNA transferred on Southern blot with heterologous pea legumin cDNA probes. Southern hybridization of restricted chickpea genomic DNA with pea legumin pDUB6 and pDUB8 cDNA probes indicated presence of pea legumin homologous sequences in chickpea. About 4-5 kb EcoRI fragment was hybridized with both the probes used. DNA corresponding to this size was isolated from the gel and used for construction of subgenomic library in lambda ZAPII vector. After amplification, library was screened for presence of legumin genes in chickpea. Out of clones screened (0.1 x 10(6)), three positive clones were obtained. Restriction analysis and hybridization studies showed presence of complete structural gene in each of the identified positive clones.
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Proteínas de Plantas / Plantas Medicinais / Biblioteca Genômica / Testes Genéticos / Genoma de Planta / Fabaceae Tipo de estudo: Estudo diagnóstico / Estudo de rastreamento Idioma: Inglês Revista: Indian J Exp Biol Ano de publicação: 1996 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Proteínas de Plantas / Plantas Medicinais / Biblioteca Genômica / Testes Genéticos / Genoma de Planta / Fabaceae Tipo de estudo: Estudo diagnóstico / Estudo de rastreamento Idioma: Inglês Revista: Indian J Exp Biol Ano de publicação: 1996 Tipo de documento: Artigo