Association of cucumovirus and potyvirus with betelvine (Piper betle L.) as evidenced by ELISA and RT-PCR.
Indian J Exp Biol
;
2003 Mar; 41(3): 273-6
Artigo
em Inglês
| IMSEAR
| ID: sea-60032
ABSTRACT
An attempt was made to detect various viruses of Piper betle grown at Mahoba and Banthara in India. DAC-ELISA and RT-PCR tests were performed in leaf sap samples of betelvine for detection of a cucumovirus (Cucumber mosaic virus) and potyvirus (Bean yellow mosaic virus) using specific antibodies and universal primers of respective viruses. DAC-ELISA could detect only CMV. However, RT-PCR detected both cucumovirus and potyvirus infection in betelvine samples. Association of CMV with betelvine was observed for the first time in the present study.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
DNA Viral
/
Ensaio de Imunoadsorção Enzimática
/
Sequência de Bases
/
Potyvirus
/
Primers do DNA
/
Cucumovirus
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Anticorpos Antivirais
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2003
Tipo de documento:
Artigo
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