Production of 2-hydroxy-4-methoxybenzaldehyde in roots of tissue culture raised and acclimatized plants of Decalepis hamiltonii Wight & Arn., an endangered shrub endemic to Southern India and evaluation of its performance vis-a-vis plants from natural habitat.
Indian J Exp Biol
;
2004 Jan; 42(1): 106-10
Artigo
em Inglês
| IMSEAR
| ID: sea-60094
ABSTRACT
Axillary buds obtained from field grown plants of D. hamiltonii were used to initiate multiple shoots on Murashige and Skoog's medium (MS) supplemented with 2 mg L(-1) 6-benzyl aminopurine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Profuse rooting was achieved when the actively growing shoots were cultured on MS medium supplemented with 1.0 mg l(-1) indole-3-butyric acid (IBA). Regenerated plants were grown successfully in the plains, in contrast to wild growth in high altitudes and rocky crevices of hilly regions. Roots of different sizes from one-year-old tissue culture raised field grown plants had the same profile of 2-hydroxy-4-methoxybenzaldehyde as that of wild plants. A maximum of 0.14% and 0.12% 2-hydroxy-4-methoxybenzaldehyde was produced in roots of one year old tissue culture derived plants and greenhouse grown plants respectively.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plantas
/
Temperatura
/
Benzaldeídos
/
Divisão Celular
/
Brotos de Planta
/
Raízes de Plantas
/
Técnicas de Cultura
/
Índia
/
Ácidos Indolacéticos
/
Cromatografia Gasosa-Espectrometria de Massas
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2004
Tipo de documento:
Artigo
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