Detergent-mediated destaining of coomassie brilliant blue-stained SDS polyacrylamide gels.
Indian J Exp Biol
;
2001 Jan; 39(1): 95-7
Artigo
em Inglês
| IMSEAR
| ID: sea-60278
ABSTRACT
A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-mediated destaining procedure, the present method is economical and user-friendly.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Corantes de Rosanilina
/
Detergentes
/
Eletroforese em Gel de Poliacrilamida
/
Indicadores e Reagentes
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2001
Tipo de documento:
Artigo
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