Fed-batch fermentation studies with Bacillus thuringiensis H-14 synthesising endotoxin.
Indian J Exp Biol
;
1991 Nov; 29(11): 1031-4
Artigo
em Inglês
| IMSEAR
| ID: sea-60289
ABSTRACT
Cell yield and toxicity of B. thuringiensis H-14 was improved markedly by adopting a simple fed-batch fermentation technique based on controlling glucose concentration. Maintenance of steady glucose concentration (0.3-0.5%) in the culture medium was achieved by the continuous addition of concentrated glucose solution. Addition of glucose at 3 g/hr/l of culture starting from 3rd hr till 16th hr of fermentation was found to yield cell densities of 80 g/l (wet weight) which represented a nearly 3-fold increase over the batch mode. A fivefold increase in toxicity was obtained by fed-batch fermentation. Cultivation of B. thuringiensis H-14 to high cell densities had no negative effect on sporulation and toxin synthesis. The rate of pH drop and dissolved oxygen level were within manageable limits.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Bacillus thuringiensis
/
Proteínas de Bactérias
/
Toxinas Bacterianas
/
Endotoxinas
/
Fermentação
/
Proteínas Hemolisinas
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1991
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS