Your browser doesn't support javascript.
loading
Fed-batch fermentation studies with Bacillus thuringiensis H-14 synthesising endotoxin.
Indian J Exp Biol ; 1991 Nov; 29(11): 1031-4
Artigo em Inglês | IMSEAR | ID: sea-60289
ABSTRACT
Cell yield and toxicity of B. thuringiensis H-14 was improved markedly by adopting a simple fed-batch fermentation technique based on controlling glucose concentration. Maintenance of steady glucose concentration (0.3-0.5%) in the culture medium was achieved by the continuous addition of concentrated glucose solution. Addition of glucose at 3 g/hr/l of culture starting from 3rd hr till 16th hr of fermentation was found to yield cell densities of 80 g/l (wet weight) which represented a nearly 3-fold increase over the batch mode. A fivefold increase in toxicity was obtained by fed-batch fermentation. Cultivation of B. thuringiensis H-14 to high cell densities had no negative effect on sporulation and toxin synthesis. The rate of pH drop and dissolved oxygen level were within manageable limits.
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Bacillus thuringiensis / Proteínas de Bactérias / Toxinas Bacterianas / Endotoxinas / Fermentação / Proteínas Hemolisinas Idioma: Inglês Revista: Indian J Exp Biol Ano de publicação: 1991 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Bacillus thuringiensis / Proteínas de Bactérias / Toxinas Bacterianas / Endotoxinas / Fermentação / Proteínas Hemolisinas Idioma: Inglês Revista: Indian J Exp Biol Ano de publicação: 1991 Tipo de documento: Artigo