Cryopreservation of mouse embryos at -196 degrees C by vitrification.
Indian J Exp Biol
;
1989 Apr; 27(4): 383-4
Artigo
em Inglês
| IMSEAR
| ID: sea-60379
ABSTRACT
Embryos (8-16 cell) were obtained from random bred albino mice (6-8 weeks old) that were induced to superovulate by injections of 5 I.U. PMSG and 5 I.U. hCG given 48 hr apart. Embryos were exposed to intracellular cryoprotecting medium (glycerol 10%, 1-2 propanediol 20% in PBS) for 10 min and then transferred to extracellular vitrification medium (25% glycerol, 25% 1-2 propanediol in PBS). Vitrification medium containing embryos, and diluent (1 M sucrose) were loaded in a straw and immediately plunged into liquid N2. After thawing at 20 degrees C, the contents of the straw were mixed by shaking (1 step dilution) and emptied in a petri dish. After 3 washings in culture medium the embryos were kept in CO2 incubator for further development. In 3-step dilution procedure the dilution of cryoprotectants was done in 0.5 and 0.25 M sucrose before culture. Embryos in 3-step dilution of cryoprotectants exhibited high survival as compared to 1-step dilution (20.23% vs 6.55%).
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Preservação de Tecido
/
Criopreservação
/
Crioprotetores
/
Embrião de Mamíferos
/
Animais
/
Camundongos
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1989
Tipo de documento:
Artigo
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