Immunological identification of two female-specific proteins from the plasma of Indian freshwater murrel, Channa punctatus (Bloch).
Indian J Exp Biol
;
2002 Mar; 40(3): 288-95
Artigo
em Inglês
| IMSEAR
| ID: sea-60418
ABSTRACT
Existence of a non-phosphorylated female-specific protein (FS II), in addition to phosphorylated vitellogenin (FS I), in the plasma of murrel by exogenous administration of estradiol-17beta is reported. Polyspecific rabbit antibodies were raised against estrogen-inducible murrel plasma proteins. This antiserum was absorbed with normal male serum in order to obtain female-specific antiserum (FSAS). Radial immunodiffusion studies suggested that both the proteins (FS I and FS II) were present in the plasma of E2-treated and normal vitellogenic females and in the ovarian homogenate from gravid females, but absent in normal male plasma. Autoradiographic experiments demonstrated that phosphorus moiety was attached with FS I only. Further, immunoelectrophoretic analysis and peptide maps supported the observation that FS I and FS II were discrete, unrelated female-specific proteins.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Perciformes
/
Feminino
/
Masculino
/
Proteínas Sanguíneas
/
Imunoquímica
/
Vitelogeninas
/
Caracteres Sexuais
/
Animais
/
Especificidade de Anticorpos
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2002
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS