Prepatent detection of Fasciola gigantica infection in bovine calves using metacercarial antigen.
Indian J Exp Biol
;
2006 Sep; 44(9): 749-53
Artigo
em Inglês
| IMSEAR
| ID: sea-60543
ABSTRACT
Metacercarial antigen of Fasciola gigantica was evaluated for early immunodiagnosis of experimental bovine fasciolosis using ELISA and Western blot. In ELISA, the experimental F. gigantica infection was detected as early as 2 weeks post-infection (WPI). The gradual increasing trend of antibody level was observed from 2 to 7 WPI, followed by a plateau, which was maintained up to 14 WPI. In Western blot, sera from experimentally infected calves recognized one distinct polypeptide of 21 kDa in fractionated metacercarial antigen as early as 10th day post infection. From 2 WPI, more polypeptide bands were reacting. Recognition of these protein bands persisted till the end of the experiment (14 WPI). Cattle sera collected from the field showed 34.5% seroprevalence of fasciolosis by ELISA using MAg. Comparative immunoblot studies of metacercarial antigen with anti-Gigantocotyle explanatum and anti-Paramphistomum epiclitum sera revealed that 21 and 25 kDa polypeptides of metacercarial antigen did not cross-react with any of these sera and appear to be unique to F. gigantica and having the desirable qualities of early and specific immunodiagnosis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Caramujos
/
Masculino
/
Bovinos
/
Ensaio de Imunoadsorção Enzimática
/
Testes Sorológicos
/
Anticorpos Anti-Helmínticos
/
Doenças dos Bovinos
/
Western Blotting
/
Modelos Animais de Doenças
/
Eletroforese em Gel de Poliacrilamida
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2006
Tipo de documento:
Artigo
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