Use of polymerase chain reaction in analysing recombinant cDNA clones encoding storage proteins of chickpea Cicer arietinum L.
Indian J Exp Biol
;
1996 Oct; 34(10): 1019-25
Artigo
em Inglês
| IMSEAR
| ID: sea-60692
ABSTRACT
A simple and reliable method was undertaken for the use of polymerase chain reaction in analyzing cDNA clones. Amplification was done of the inserts from positive legumin clones isolated from a cDNA library constructed from developing chickpea cotyledons in the expression vector, gt11. Amplification was made simple by using oligonucleotide primers which allowed convenient sizing, subcloning and sequencing of inserts by di-deoxy chain termination method. This simple method may provide opportunity to isolate large number of agronomically important genes from gene libraries.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas de Plantas
/
Plantas Medicinais
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
Homologia de Sequência de Aminoácidos
/
DNA Complementar
/
DNA de Plantas
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1996
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS