Non-chemical method of DNA recovery and characterization of Mycobacterium avium subspecies paratuberculosis using IS 900 PCR.
Indian J Exp Biol
;
2007 Sep; 45(9): 812-6
Artigo
em Inglês
| IMSEAR
| ID: sea-60739
ABSTRACT
In the present study, two methods of DNA isolation-routine, traditional and standard DNA isolation protocol for Mycobacteria (Method 1) and a new non-chemicals and non-enzymes (physical) method (Method 2) of DNA recovery have been compared and evaluated in IS900 PCR for the specific detection of pathogen. Using the new Method 2, DNA has been recovered from few (1 - 3 colonies), extremely minute and stunted colonies. DNA, thus, isolated from these colonies (colonies PCR) and cultured for the first time from the cases of Crohn's disease in human beings, dairy cattle, raw milk and pasteurized commercial milk samples has been characterized in the present study. It is the first report from India.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
DNA Bacteriano
/
Bovinos
/
Reação em Cadeia da Polimerase
/
Técnicas Bacteriológicas
/
Mycobacterium avium subsp. paratuberculosis
/
Animais
Tipo de estudo:
Estudos de avaliação
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2007
Tipo de documento:
Artigo
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