In vitro mass multiplication of Ophiorrhiza mungo Linn.
Indian J Exp Biol
;
2004 Jun; 42(6): 639-42
Artigo
em Inglês
| IMSEAR
| ID: sea-60740
ABSTRACT
A protocol for in vitro mass multiplication of plants through seedling (shoot) cultures was established for Ophiorrhiza mungo. Maximum number of adventitious shoots per shoot culture (10.4 +/- 1.72) was initiated on MS solid medium supplemented with BAP (2.22 microM) after 3 weeks. Shoots were further multiplied (12.8 +/- 2.8) through subculture of intact shoots and reculture of nodal segments of aseptic shoots (6.5 +/- 0.94) in MS solid medium containing BAP (0.89 microM). Shoot elongation (1.27 +/- 0.12 cm) was achieved in the medium containing GA3 (1.44 microM) in two weeks. Rooting was favoured in basal agar medium supplemented with IBA (12.3 microM) plus NAA (1.07 microM). The plants were successfully established (100%) in the pots containing sand and top soil (11) mixture in a period of two weeks.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Reguladores de Crescimento de Plantas
/
Fatores de Tempo
/
Divisão Celular
/
Dióxido de Silício
/
Brotos de Planta
/
Raízes de Plantas
/
Fenômenos Fisiológicos Vegetais
/
Rubiaceae
/
Meios de Cultura
/
Ágar
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2004
Tipo de documento:
Artigo
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