Attachment of spermatozoa to in vitro matured bovine oocytes in TCM-199 and MEM media.
Indian J Exp Biol
;
1998 Apr; 36(4): 367-70
Artigo
em Inglês
| IMSEAR
| ID: sea-60989
ABSTRACT
Investigation was undertaken to study the efficacy of TCM 199 and MEM media in the process of oocyte maturation and fertilization after harvesting the oocytes from slaughtered cow and heifer ovaries by aspiration and mincing techniques. Out of 354 number of ovaries, mincing method could yield 1.35 (50.98%) of good quality oocytes per ovary as comparison to 0.61 (55.45%) by aspiration method. The TCM 199 and MEM could record the maturation of 85.71% and 60.26% of oocytes and the rate of sperm penetration was higher in TCM 199 matured oocytes (97.21%) as compared to MEM matured ones (74.46%). The staining of oocytes by aceto-orcein stain was also employed.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Oócitos
/
Interações Espermatozoide-Óvulo
/
Espermatozoides
/
Feminino
/
Masculino
/
Bovinos
/
Fertilização in vitro
/
Meios de Cultura
/
Animais
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1998
Tipo de documento:
Artigo
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