Effect of sodium on Saccharomyces cerevisiae invertase activity.
Indian J Exp Biol
;
1998 May; 36(5): 493-6
Artigo
em Inglês
| IMSEAR
| ID: sea-61012
ABSTRACT
Effect of Na+ ions on yeast invertase activity has been studied as a function of pH. At acidic pH, Na+ (5-100 mM) had no effect but invertase activity was inhibited (38-44%) by Na+ ions (100 mM) with an increase in pH (6.8 and 8.0). Kinetic analysis revealed that invertase inhibition by Na+ ions was non-competitive and reversible in nature. Value of K(m) remained unaltered (33.3 mM) in presence of Na+ (20-100 mM) while Vmax decreased by 21-44% under these conditions. Value of Ki was of the order of 85 mM. Mechanism of observed inhibition of invertase activity as a consequence of Na+ ions interactions at the active site of the enzyme has been described.
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DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Saccharomyces cerevisiae
/
Sódio
/
Cinética
/
Beta-Frutofuranosidase
/
Glicosídeo Hidrolases
/
Concentração de Íons de Hidrogênio
/
Soros Imunes
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1998
Tipo de documento:
Artigo
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