In vivo visualization of bonghan ducts inside blood vessels of mice by using an Alcian blue staining method.
Indian J Exp Biol
;
2008 May; 46(5): 336-9
Artigo
em Inglês
| IMSEAR
| ID: sea-61732
ABSTRACT
An in vivo method using Alcian blue (AB) was developed for visualizing floating threadlike tissues inside blood vessels of mice. These novel structures called intravascular Bonghan ducts (IBHDs) are considered as extension of acupuncture meridians. For in vivo imaging of IBHDs, AB solution (pH 7.4) that stains mucopolysaccharides like hyaluronic acid was used. After injecting AB solution into the femoral vein of a mouse, the threadlike structures, stained deep blue, inside the inferior vena cava. The histological results, such as hematoxylin, eosin, and AB staining, show the compositions of the cells and the extracellular matrix in the IBHD. Further studies are needed to investigate their physiological functions, especially in relation with those of other circulatory systems.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Propídio
/
Biofísica
/
Vasos Sanguíneos
/
Masculino
/
Microscopia de Contraste de Fase
/
Amarelo de Eosina-(YS)
/
Microscopia Confocal
/
Azul Alciano
/
Veia Femoral
/
Hematoxilina
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2008
Tipo de documento:
Artigo
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