H2O2-induced lipid peroxidation in mitochondria of pulmonary vascular smooth muscle tissue and its modification by DFO, DMTU and DIDS.
Indian J Exp Biol
;
1996 Dec; 34(12): 1220-3
Artigo
em Inglês
| IMSEAR
| ID: sea-61740
ABSTRACT
Treatment of bovine pulmonary artery smooth muscle tissue mitochondria with H2O2 stimulated iron release, hydroxyl radical (OH) production and lipid peroxidation. Pretreatment of mitochondria with deferoxamine (DFO) and dimethyl thiourea (DMTU) prevented OH production and markedly reduced lipid peroxidation without appreciably altering iron release caused by H2O2. Simultaneous treatment of either DFO or DMTU with H2O2 significantly reduced lipid peroxidation and also prevented OH production without causing marked decrease in iron release. In contrast, addition of DFO or DMTU even 2 min after treatment of the mitochondria with H2O2 did not significantly altered iron release, OH production and lipid peroxidation. Pretreatment of the mitochondria with 4,4'-dithiocyano-2,2'-disulfonic acid stilbene (DIDS) markedly reduced lipid peroxidation without appreciably altering the increase in OH production and iron release caused by H2O2.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tioureia
/
Bovinos
/
Peroxidação de Lipídeos
/
Circulação Pulmonar
/
Sequestradores de Radicais Livres
/
Ácido 4,4'-Di-Isotiocianoestilbeno-2,2'-Dissulfônico
/
Desferroxamina
/
Peróxido de Hidrogênio
/
Animais
/
Mitocôndrias Musculares
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1996
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS