Heat modifiability of outer membrane protein of Pasteurella multocida serotype B:2.
Indian J Exp Biol
;
2002 Jan; 40(1): 106-8
Artigo
em Inglês
| IMSEAR
| ID: sea-61892
ABSTRACT
Outer membrane proteins (OMP) are generally porins, functioning as molecular sieves assisting in the transmembrane transportation. Heat modifiable characteristics of OMP from P. multocida B 2 have been explored to know their basic characteristics on event of temperature rise. A major band of 32 kDa and two minor bands of approximately 39 and approximately 28 kDa were found to be heat modifiable. It is suggested that boiling at 100 degrees C in presence of beta mercaptoethanol for 5 min is sufficient for characterisation of OMP by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Pasteurella multocida
/
Eletroforese em Gel de Poliacrilamida
/
Temperatura Alta
/
Animais
/
Camundongos
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2002
Tipo de documento:
Artigo
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