Cell population growth in chick blastoderms cultured in vitro.
Indian J Exp Biol
;
1990 Jun; 28(6): 526-30
Artigo
em Inglês
| IMSEAR
| ID: sea-61957
ABSTRACT
Primitive streak stage chick blastoderms were cultured in vitro up to 30 hr by New's technique. Chick blastoderms reaching stages 4 to 12 in vitro cultures and in ovo were harvested and homogenized to release cell nuclei. Fluorescent ethidium bromide-stained nuclei in homogenates were counted in Neubauer's chamber and the size of total blastoderm cell population was determined. Linear regression analysis revealed that both in ovo and in vitro chick blastoderm cell population grows in a biphasic manner with comparable cell population doubling times and the morphogenesis is not affected in vitro during the culture period.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Blastoderma
/
Contagem de Células
/
Divisão Celular
/
Células Cultivadas
/
Embrião de Galinha
/
Análise de Regressão
/
Animais
/
Microscopia de Fluorescência
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1990
Tipo de documento:
Artigo
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