Effects of different factors on immature embryo culture, PLBs differentiation and rapid mass multiplication of Coelogyne suaveolens (Lindl.) Hook.
Indian J Exp Biol
;
2008 Apr; 46(4): 243-8
Artigo
em Inglês
| IMSEAR
| ID: sea-62301
ABSTRACT
In vitro mass production of C. suaveolens (Lindl.) Hook, an endangered orchid with its snowy white flowers having horticultural potential was accomplished through immature seed culture, and subsequent plant regeneration. The developmental stage of the immature seeds and nutrient media significantly influenced the germination frequency. Seeds at 13 months after pollination cultured on 3% sucrose containing Murashige and Skoog (MS) medium with 9 microM alpha-naphthaleneacetic acid (NAA), and 15% coconut water exhibited 93% germination after 40 days of culture. Upon subculture, the germinated shoots on MS medium with 9 microM BA, 6 microM NAA, 3% casein hydrolysate and 0.1% activated charcoal (AC) yielded >12 shoots per shoot or bud. Addition of AC favoured the enlargement of pseudobulbs and better rooting. The plantlets transferred to community potting mix after in vitro hardening (8-10 wk) displayed 85% survival.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Reguladores de Crescimento de Plantas
/
Sementes
/
Germinação
/
Biomassa
/
Meios de Cultura
/
Orchidaceae
/
Técnicas de Cultura
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2008
Tipo de documento:
Artigo
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