An enzyme linked immunosorbent assay for monitoring Bacillus sphaericus toxin.
Indian J Exp Biol
;
1991 Oct; 29(10): 953-7
Artigo
em Inglês
| IMSEAR
| ID: sea-62466
ABSTRACT
Larvicidal proteins of B. sphaericus H5a5b (strain VCRC B42), purified by ion-exchange chromatography were used to raise antibodies in rabbits. The antibodies were specific in reacting to alkali-solubilized fractions from whole cells of toxic strains only. Ouchterlony immunodiffusion showed homology in toxin structure between strains of different serotype. A sandwich ELISA using avidin-biotin amplification was standardized. The lowest detectable limit was 6.25 ng/ml. Near linear quantitative binding of the antigen was found in the range 25-200 ng/ml. The growth, toxin level and LC50 values during various stages of fermentation of B. sphaericus strains 1593 and B42 were compared. There was significant correlation between LC50 values and toxin levels as measured by ELISA.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Bacillus
/
Toxinas Bacterianas
/
Ensaio de Imunoadsorção Enzimática
/
Western Blotting
/
Cromatografia por Troca Iônica
/
Imunodifusão
/
Fermentação
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1991
Tipo de documento:
Artigo
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