Mechanism of nickel resistance in a cobalt-resistant wall-less mutant of Neurospora crassa (fz; sg; os-1).
Indian J Exp Biol
;
2004 Nov; 42(11): 1117-22
Artigo
em Inglês
| IMSEAR
| ID: sea-62559
ABSTRACT
A cobalt-resistant wall-less mutant of N. crassa (Cor-sl) characterized previously was also found to be 3-fold more resistant to nickel when compared to the parent wall-less mutant (W-sl). The Cor-sl strain accumulates relatively lower amounts of nickel when compared to W-sl. Sub-cellular fractionation showed significant quantities of nickel to be associated with nuclear and mitochondrial fractions in both the wall-less mutants. However significant differences were observed in vacuolar fractions of W-sl and Cor-sl strains. Fractionation of cell-free extracts on Sephadex G-10 column resolved nickel into two peaks, of which the peak II in Cor-sl constituted 70% of nickel, while the same in W-sl was about 30%. A 3-fold increase in histidine content was observed in case of Cor-sl as compared to W-sl strain, suggesting its role in Ni-resistance.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Frações Subcelulares
/
Parede Celular
/
Cobalto
/
Farmacorresistência Fúngica
/
Histidina
/
Mutação
/
Neurospora crassa
/
Níquel
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2004
Tipo de documento:
Artigo
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