Antimicrobial protein from Streptomyces fulvissimus inhibitory to methicillin resistant Staphylococcus aureus.
Indian J Exp Biol
;
2008 Apr; 46(4): 254-7
Artigo
em Inglês
| IMSEAR
| ID: sea-62570
ABSTRACT
Fermented culture of Streptomyces fulvissimus was found to secrete an antibacterial protein inhibitory to Micrococcus luteus, Bacillus subtilis, Bacillus cereus and methicillin resistant Staphylococcus aureus (MRSA) strains. The extracellular protein from the fermented culture on concentration revealed a high molecular weight peptide of 63kDa on SDS-PAGE gel and the region on gel displayed inhibitory activity against methicillin resistant Staphylococcus aureus. Bioactivity of the extra cellular protein was non-sensitive to proteinase K, alpha chymotrypsin, protease, EDTA (ethylene diamine tetra acetic acid), PMSF (phenyl methyl sulfonyl fluoride) and DMSO (dimethyl sulfoxide) but partially susceptible to amylase and heat. Glycoprotein nature of the proteinaceous compound was confirmed by periodic acid schiffs (PAS) staining. The secretary protein of S. fulvissimus demonstrated a significant activity against MRSA strain. It could be an important source for developing new drugs to control multidrug resistant gram positive bacteria.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Staphylococcus aureus
/
Streptomyces
/
Proteínas de Bactérias
/
Testes de Sensibilidade Microbiana
/
Resistência a Meticilina
/
Eletroforese em Gel de Poliacrilamida
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Fermentação
/
Antibacterianos
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2008
Tipo de documento:
Artigo
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