In vitro regeneration of Hypericum patulum Thunb.--a medicinal plant.
Indian J Exp Biol
;
2001 Sep; 39(9): 947-9
Artigo
em Inglês
| IMSEAR
| ID: sea-62624
ABSTRACT
A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot formation. Upon transfer to phytohormone free liquid medium following a brief exposure to auxin, root formation occurred from the micro shoots . Rooted plants were hardened and transferred to soil. Regeneration potentiality was found to be constant throughout the year in long term cultures.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ácido Pantotênico
/
Reguladores de Crescimento de Plantas
/
Plantas Medicinais
/
Purinas
/
Regeneração
/
Tiamina
/
Biotina
/
Adenina
/
Brotos de Planta
/
Raízes de Plantas
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2001
Tipo de documento:
Artigo
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