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In vitro clonal propagation of Chlorophytum borivilianum Sant. et Fernand., a rare medicinal herb from immature floral buds along with inflorescence axis.
Indian J Exp Biol ; 2006 Jan; 44(1): 77-82
Artigo em Inglês | IMSEAR | ID: sea-63076
ABSTRACT
A novel method of shoot regeneration from immature floral buds along with inflorescence axis in C. borivilianum, a rare medicinal herb is described. Using this explant, axenic cultures were established with very less contamination (10%). MS medium with 2 mg l(-1) kinetin and 0.1 mg l(-1) 2, 4-dichlorophenoxyacetic acid proved to be the best for multiple shoot induction. Maximum number (35) of shoot production was achieved in MS medium with 2 mg l(-1) benzylaminopurine. Rooting of shoots (86.7%) with maximum fasciculated roots (5) occurred on Knops medium containing iron and vitamins of MS medium with 2 mg l(-1) indole-3-butyric acid and 0.1% activated charcoal. Plant survival was 80% in four weeks after their removal from in vitro conditions. Per explant 34 hardened plants generated within 50 weeks. This protocol can be useful for large-scale clonal multiplication from immature floral buds with inflorescence axis and successfully used for germplasm conservation of this rare medicinal herb without destroying the mother plant.
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Plantas Medicinais / Brotos de Planta / Raízes de Plantas / Liliaceae / Flores / Técnicas de Cultura Idioma: Inglês Revista: Indian J Exp Biol Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Plantas Medicinais / Brotos de Planta / Raízes de Plantas / Liliaceae / Flores / Técnicas de Cultura Idioma: Inglês Revista: Indian J Exp Biol Ano de publicação: 2006 Tipo de documento: Artigo