In vitro clonal propagation of Chlorophytum borivilianum Sant. et Fernand., a rare medicinal herb from immature floral buds along with inflorescence axis.
Indian J Exp Biol
;
2006 Jan; 44(1): 77-82
Artigo
em Inglês
| IMSEAR
| ID: sea-63076
ABSTRACT
A novel method of shoot regeneration from immature floral buds along with inflorescence axis in C. borivilianum, a rare medicinal herb is described. Using this explant, axenic cultures were established with very less contamination (10%). MS medium with 2 mg l(-1) kinetin and 0.1 mg l(-1) 2, 4-dichlorophenoxyacetic acid proved to be the best for multiple shoot induction. Maximum number (35) of shoot production was achieved in MS medium with 2 mg l(-1) benzylaminopurine. Rooting of shoots (86.7%) with maximum fasciculated roots (5) occurred on Knops medium containing iron and vitamins of MS medium with 2 mg l(-1) indole-3-butyric acid and 0.1% activated charcoal. Plant survival was 80% in four weeks after their removal from in vitro conditions. Per explant 34 hardened plants generated within 50 weeks. This protocol can be useful for large-scale clonal multiplication from immature floral buds with inflorescence axis and successfully used for germplasm conservation of this rare medicinal herb without destroying the mother plant.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plantas Medicinais
/
Brotos de Planta
/
Raízes de Plantas
/
Liliaceae
/
Flores
/
Técnicas de Cultura
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2006
Tipo de documento:
Artigo
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