A simple method for efficient extraction and purification of C-phycocyanin from Spirulina platensis Geitler.
Indian J Exp Biol
;
2005 Mar; 43(3): 277-9
Artigo
em Inglês
| IMSEAR
| ID: sea-63141
ABSTRACT
Phycocyanin is a major light harvesting accessory pigment of red algae and cyanobacteria. In the light of its many commercial applications in food and pharmaceutical industry, purity of the pigment plays a major role. Pharmaceutical industry demands a highly pure phycocyanin with A620/280 ratio of 4 and food industry a ratio of 2. In the present study phycocyanin was extracted in sodium phosphate buffer (pH 7) after macerating in liquid nitrogen. The crude phycocyanin thus extracted was precipitated with 50% ammonium sulphate, purified by dialysis and finally by gel filtration chromatography. Pure phycocyanin was finally obtained with an A620/A280 value of 4.98.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ficocianina
/
Espectrofotometria
/
Proteínas de Bactérias
/
Bioquímica
/
Extratos Vegetais
/
Cromatografia em Gel
/
Cianobactérias
/
Rodófitas
/
Eletroforese em Gel de Poliacrilamida
/
Spirulina
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2005
Tipo de documento:
Artigo
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