Detection of superoxide dismutase isozymes in normal and X-irradiated chick amniotic fluid by using isoelectric focussing.
Indian J Exp Biol
;
1996 Sep; 34(9): 901-4
Artigo
em Inglês
| IMSEAR
| ID: sea-63388
ABSTRACT
Isoelectric focussing of amniotic fluid of chick system over pH gradient of 4-6 revealed presence of 8 superoxide dismutases. The superoxide dismutase (SOD) isozymes were identified as one major isozyme and other seven were minor ones. Higher expression of SOD (major isozyme) with exposure of 1000 R was studied. The isoelectric point (pI) of major SOD isozyme in control (5) differs from radiation exposed samples (5.12). Hence in radiation exposed amniotic fluid showed more pronounced SOD major isozyme, than control amniotic fluid. It is clearly evident that the SOD isozyme with X-irradiation having different isoelectric points, may lead to modification of the amino acid composition and charges of amino acids in the protein structure of SOD. So, the higher SOD isozyme expression has a role in defence action against free radical damage by X-irradiation during embryonic development.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Superóxido Dismutase
/
Raios X
/
Embrião de Galinha
/
Líquido Amniótico
/
Focalização Isoelétrica
/
Isoenzimas
/
Animais
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
1996
Tipo de documento:
Artigo
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