Coordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats.
Artigo
em Inglês
| IMSEAR
| ID: sea-64211
ABSTRACT
BACKGROUND:
Fat feeding increases the activity of intestinal alkaline phosphatase in the serum. The mechanism underlying this increase is unknown. Surfactant-like particles (SLP) secreted by enterocytes have been implicated in this phenomenon.OBJECTIVE:
To study the effect of feeding fish oil and protein synthesis inhibitors on alkaline phosphatase activity in serum and in different intestinal fractions.METHODS:
Male albino rats were fed 2 mL of fish oil and were injected cycloheximide or actinomycin D. Alkaline phosphatase activity was determined in the serum and intestinal fractions (SLP, mucosa, muscularis).RESULT:
Feeding fish oil significantly elevated alkaline phosphatase activity in serum (p< 0.001) and intestinal mucosa (p< 0.01). Administration of cycloheximide or actinomycin D significantly reduced alkaline phosphatase activity in serum (p< 0.01) and in intestinal mucosa (p< 0.05). BCIP staining of brush border alkaline phosphatase activity in acrylamide gels yielded similar results.CONCLUSIONS:
These findings suggest a co-ordination between alkaline phosphatase synthesis and its assembly into lipoprotein vesicles, such as SLP, secreted by enterocytes in response to fat feeding.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ratos
/
Ratos Endogâmicos
/
Valores de Referência
/
Masculino
/
Gorduras na Dieta
/
Taxa de Depuração Metabólica
/
Probabilidade
/
Sensibilidade e Especificidade
/
Modelos Animais de Doenças
/
Fosfatase Alcalina
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Ano de publicação:
2004
Tipo de documento:
Artigo
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