Development of an animal model of hepatic fibrosis by excretory-secretory antigen of Ascaris suum.
Artigo
em Inglês
| IMSEAR
| ID: sea-64500
ABSTRACT
OBJECTIVE:
The excretory-secretory (ES) antigens of Ascaris suum are known to cause hepatic damage in animals. The present study was aimed at developing an animal model of hepatic fibrosis with these antigens.METHODS:
Three doses of ES antigens of A. suum were injected into 24 golden hamsters on days 0, 10 and 20. Batches of 8 animals each were sacrificed at 3 days, 45 days and 90 days after the third injection, after collection of blood. Three groups of 6 control animals each were injected with normal saline and were sacrificed similarly. Liver biochemistry, leukocyte migration inhibition test on cells separated from spleen, and liver histology were carried out.RESULTS:
Serum ALT levels in experimental animals were significantly higher than those in control animals at days 3, 45 and 90 after the last antigen dose; AST levels were elevated 45 and 90 days after the last dose of ES antigen. Leukocyte migration inhibition in experimental animals was 58.2 (8.5)%, 51.6 (11.2)% and 50.5 (12.8)% at days 3, 45 and 90 after the last antigen dose. Marked centrivenular degeneration and necrosis were observed in liver tissue in all the experimental animals sacrificed 72 h after the last antigen dose. Condensation of reticulin around the portal zone with extension into the liver lobule was observed in 4 of 8 and 7 of 8 experimental animals sacrificed 45 and 90 days, respectively, after the last dose. Control animals did not have such lesions.CONCLUSION:
An animal model of hepatic fibrosis could be produced by repeated injection of ES antigens of A. suum.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Valores de Referência
/
Masculino
/
Sensibilidade e Especificidade
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Cricetinae
/
Ascaris suum
/
Modelos Animais de Doenças
/
Contagem de Leucócitos
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Fígado
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Cirrose Hepática
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Testes de Função Hepática
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Ano de publicação:
2000
Tipo de documento:
Artigo
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