Isoenzyme and molecular characterization of Entamoeba histolytica and Entamoeba dispar isolates from symptomatic and asymptomatic subjects.
Artigo
em Inglês
| IMSEAR
| ID: sea-64776
ABSTRACT
AIM:
To correlate the clinical features of amebic infections with the characteristics of Entamoeba culture isolates of stools.METHODS:
Isolates from seven irritable bowel syndrome (IBS) patients, four asymptomatic cyst passers (ACP) and five patients with invasive amebic disease were subjected to hexokinase polyacrylamide electrophoresis (HK-PAGE) and their DNA subjected to restriction fragment (RF) analysis of amplified polymerase chain reaction (PCR) products. These findings were correlated with anti-amebic serology. Two axenic pathogenic strains (HM1IMSS, NIH200) and one xenic nonpathogenic strain (SAW1734) were used as standards.RESULTS:
All isolates from IBS patients as well as ACP had slow-moving (nonpathogenic) band pattern, whereas those from patients with invasive disease had fast-moving (pathogenic) band pattern on HK-PAGE. Serological data using EIA and RF patterns of PCR-amplified genome corroborated these results.CONCLUSIONS:
Our results support the view that there are two species of Entamoeba infecting humans--E. histolytica(pathogenic) and E. dispar (nonpathogenic), and HK-PAGE of culture isolates can differentiate between them.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
Humanos
/
Doenças Inflamatórias Intestinais
/
DNA de Protozoário
/
Entamoeba
/
Entamoeba histolytica
/
Entamebíase
/
Hexoquinase
/
Enteropatias
/
Isoenzimas
Tipo de estudo:
Guia de Prática Clínica
Idioma:
Inglês
Ano de publicação:
1999
Tipo de documento:
Artigo
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