Detection of HPV DNA in cervical carcinomas by PCR and hybrid capture assay.
Indian J Pathol Microbiol
;
2003 Oct; 46(4): 596-9
Artigo
em Inglês
| IMSEAR
| ID: sea-72708
ABSTRACT
HPV DNA was detected in exfoliated cervical cells of 73% (85/116) cervical cancer patients by PCR using HPV consensus primers and by hybrid capture assay (HC II) (Digene Corp., USA) in 77 of the 85 cases found HPV positive by PCR. Presence of HPV 16/18 DNA were investigated in the 79 cases by PCR using type specific primers. HPV 16 was detected in 31 (39%) patients, HPV 18 in 7 (8.8%), both HPV 16 and 18 in 19 (24%) and HPVs other than 16/18 in 22 (27.8%) cases. Age and clinical stages had no significant effect on HPV prevalence. Double infection of HPV 16 and 18 was significantly (p<0.05) high in the older patients (56 years or more) compared to younger group. Results indicated that cervical cancers in India are strongly associated with high-risk type HPV infection. HC II assays and PCR results for detection of HPV in cervical smears were comparable.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Papillomaviridae
/
Feminino
/
Humanos
/
DNA Viral
/
Ensaio de Imunoadsorção Enzimática
/
Sequência de Bases
/
Sondas de DNA de HPV
/
Reação em Cadeia da Polimerase
/
Adulto
/
Índia
Tipo de estudo:
Estudo diagnóstico
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Indian J Pathol Microbiol
Ano de publicação:
2003
Tipo de documento:
Artigo
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