Changing patterns and re-distribution of antigen in poorly differentiated carcinomas: its implications in tumour diagnosis.
Indian J Pathol Microbiol
;
1998 Jan; 41(1): 55-66
Artigo
em Inglês
| IMSEAR
| ID: sea-73405
ABSTRACT
The variability of expression of tumour-associated antigens via either antigenic heterogeneity or antigenic modulation presents a basic problem in immunohistochemical diagnosis of poorly/undifferentiated tumours. This work was designed to study antigenic expression on human resected epithelial tumours by a panel of most widely used antibodies (EMA, CEA, AUAI & Cytokeratin) in relation to tumour differentiation and polarization. It was observed that poorly differentiated carcinoma with loss of polarity show homogeneous membrane staining (with antibodies against EMA, CEA & AUAI) in contrast to either apical (luminal) or basolateral membrane staining in well differentiated counterparts. Biochemical studies have shown that apical and basolateral epithelial cell membrane domains have a characteristic set of glycoproteins. Tight junctions are essential for maintaining this functional polarization. It was concluded that structural and functional abnormalities of tight junctions in poorly differentiated carcinomas results in loss of polarity with progressive invasion of the cell surface by antigenic glycoprotein and resultant homogeneous individual cell antigenic expression in poorly differentiated carcinomas. This study demonstrates that antigenic expression on tumour cells is not static, but dynamic and heterogeneity of antigenic expression may well be due to biological factors such as spatial configuration of the lesion.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Coloração e Rotulagem
/
Humanos
/
Imuno-Histoquímica
/
Células Tumorais Cultivadas
/
Adenocarcinoma
/
Biomarcadores Tumorais
/
Diferenciação Celular
/
Polaridade Celular
/
Epitélio
/
Anticorpos Monoclonais
Idioma:
Inglês
Revista:
Indian J Pathol Microbiol
Ano de publicação:
1998
Tipo de documento:
Artigo
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