Autofluorescence: a screening test for mycotic infection in tissues.
Indian J Pathol Microbiol
;
2008 Apr-Jun; 51(2): 215-7
Artigo
em Inglês
| IMSEAR
| ID: sea-74030
ABSTRACT
Fungal infection is a major health concern as the clinical features are not very distinctive. Lack of rapid diagnostic techniques results in delay in diagnosis, which may even culminate in a fatal outcome. The fact that many pathogenic fungal organisms autofluoresce in hematoxylin and eosin (H and E)-stained sections under ultraviolet illumination led us to evaluate the role of autofluorescence as a rapid screening technique for fungal infections. The aim of the present study was to assess the value of autofluorescence as a screening method for detecting fungi on tissue sections and to compare the results of autofluorescence with conventional histochemical stains for fungi. Hematoxylin and eosin-stained slides of mycotic lesions were examined under fluorescent microscope and the findings were compared with results of Gomori's methenamine silver and periodic acid-Schiff stains. We found fungal autofluorescence in 63 out of 64 cases studied, with a sensitivity of 97.8% and specificity of 100% in comparison with fungal stains. This was statistically significant (P < 0.05). We conclude that autofluorescence can be used as a rapid screening method for identification of fungi in tissue sections as it does not require any other specialized staining procedure.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Aspergilose
/
Coloração e Rotulagem
/
Humanos
/
Candidíase
/
Estudos Retrospectivos
/
Sensibilidade e Especificidade
/
Zigomicose
/
Nefropatias
/
Microscopia de Fluorescência
/
Micologia
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
/
Estudo observacional
/
Estudo prognóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Indian J Pathol Microbiol
Ano de publicação:
2008
Tipo de documento:
Artigo
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