Diagnostic value of in situ Polymerase Chain Reaction in leprosy.
Indian J Pediatr
;
2005 Dec; 72(12): 1043-6
Artigo
em Inglês
| IMSEAR
| ID: sea-78664
ABSTRACT
OBJECTIVE:
This prospective study was carried out to assess the diagnostic value of in situ Polymerase Chain Reaction in leprosy, particularly in enhancing the histopathological diagnosis.METHOD:
Clinical examination of 20 patients (< 16 yr) was done and skin smear for AFB was prepared. Biopsy of lesion site was taken for histopathological examination and in situ PCR testing.RESULTS:
The histopathological examination confirmed the clinical diagnosis in 45% cases only; non-specific histopathology was reported in the remaining 55% cases. In situ PCR showed a positivity of 57.1% in early/localized form of leprosy (IIBT) and 61.5% in (BB/BL) group. When compared to histopathology examination, a significant enhancement of 15% in diagnosis was seen. With in situ PCR, the diagnosis could be confirmed in 4/11 (36.3%) cases with non-specific histopathological features, (which is common in early disease) in addition to confirmation of 8/9 (88.8%) histopathologically-confirmed tissue sections. Histopathology and in situ PCR, combined together, confirmed the diagnosis in 13/20 cases (65% of total cases).CONCLUSION:
Thus, in situ PCR is an important diagnostic tool especially in early and doubtful cases of leprosy.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Pele
/
Feminino
/
Humanos
/
Masculino
/
DNA Bacteriano
/
Criança
/
Pré-Escolar
/
Reação em Cadeia da Polimerase
/
Estudos Prospectivos
/
Adolescente
Tipo de estudo:
Estudo observacional
Idioma:
Inglês
Revista:
Indian J Pediatr
Ano de publicação:
2005
Tipo de documento:
Artigo
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