Diagnosis of tuberculosis by polymerase chain reaction.
Indian J Pediatr
;
1990 Sep-Oct; 57(5): 673-7
Artigo
em Inglês
| IMSEAR
| ID: sea-79290
ABSTRACT
For the diagnosis of extrapulmonary tuberculosis in adults and all forms of tubercular infections in children, microscopic and cultural techniques have been shown to be inadequate. Many serological techniques have been employed for non culture diagnosis of tuberculosis. Early promising results have repeatedly given way to subsequent findings of non-specificity. Major mycobacterial antigens have been shown to be heat shock proteins which are highly conserved in nature. DNA probes for tuberculosis are specific but have a sensitivity equivalent to AFB smear examination. Polymerase Chain Reaction (PCR) with its ability to selectively amplify DNA fragments of interest offers a potentially powerful technique for the rapid, specific and sensitive diagnosis of tuberculosis. Samples from partially treated patients could be culture negative but can be detected by PCR.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tuberculose
/
Humanos
/
Sondas de DNA
/
Células Cultivadas
/
Criança
/
Reação em Cadeia da Polimerase
/
Adulto
/
Mycobacterium tuberculosis
Idioma:
Inglês
Revista:
Indian J Pediatr
Ano de publicação:
1990
Tipo de documento:
Artigo
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