Evaluation of purified protein derivative in the laboratory diagnosis of tuberculous meningitis.
Artigo
em Inglês
| IMSEAR
| ID: sea-95190
ABSTRACT
An enzyme-linked immunosorbent assay (ELISA) was standardised for the quantitation of IgG antibody in cerebrospinal fluid (CSF) specimens of patients with tuberculous meningitis (TBM). Purified protein derivative (PPD1) from H37Ra M tuberculosis was used as the antigen in the assay. The sensitivity of the ELISA with this antigen was evaluated in the CSF of 10 culture positive and 40 culture negative patients with TBM. The specificity of the assay was evaluated in the CSF of 50 patients with non-tuberculous neurological diseases (control group). The results obtained with this antigen were compared with commercially available tuberculin purified protein derivative (PPD2) and BCG antigens. PPD2 gave false negative results (50%) in culture positive patients with TBM, and BCG antigen gave false positive results in 32% of non-tuberculous subjects. PPD1 gave a sensitivity of 60% in culture negative patients with TBM and no false positive reactions in the non-tuberculous group. PPD1 antigen, in contrast to other mycobacterial antigens, can be very easily prepared in any routine laboratory, and this antigen is recommended for use as an aid in the laboratory diagnosis of TBM, particularly in culture negative patients with TBM.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tuberculina
/
Humanos
/
Ensaio de Imunoadsorção Enzimática
/
Proteínas do Líquido Cefalorraquidiano
/
Estudos Retrospectivos
/
Índia
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
País/Região como assunto:
Ásia
Idioma:
Inglês
Ano de publicação:
1992
Tipo de documento:
Artigo
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