Genetic analysis of pertussis strain used for vaccine production in China in complete map genome level / 中国生物制品学杂志

ABSTRACT

@#Objective To carry out genetic analysis of complete map genome of strains used for pertussis vaccine production in China.MethodsThe complete map genome of pertussis vaccine original seed for production use was sequenced by combining PacBio Sequel third-generation sequencing platform and Illumina second-generation sequencing platform,and the genome components analysis,gene function annotation and comparative genome analysis were performed. Simultaneously,the genetic stability of different generations of strains in the production process of enterprises was analyzed.Results The complete map genome of the original strain of pertussis vaccine and its different generations in the production process was obtained,with the genome length of 4. 1 Mb,the GC content of 68%,and the stable genome structure among different generations. Immunogenic or potentially immunogenic genes such as pt,pha,prn,fim2,fim3,act,tct,ompA,BrKA,rplD and BP1569 were obtained and analyzed,and no occured in different generations of genes.ConclusionIn this study,the complete map genome of pertussis strains for vaccine production with complete structure and clear background was obtained,which provided a basis for the quality control of pertussis strains for vaccine production in China,and also provided a reference for the study of molecular epidemiology of pertussis.