Isolation and Partial Characterization of Hemin-binding Cell Envelope Proteins from Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
; : 155-165, 2006.
Article
em Ko
| WPRIM
| ID: wpr-10073
Biblioteca responsável:
WPRO
ABSTRACT
The results of this study confirm that the availability of hemin influences the expression of selected membrane proteins of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens. A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is hemin regulated was identified and purified in P. gingivalis. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when P. gingivalis cells were grown under hemin-limited conditions. A 50 kDa cell envelope associated protein, whose expression is hemin regulated, is considered to be a putative hemin binding protein from P. intermedia and P. nigrescens, respectively. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein from P. gingivalis revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins. N-terminal amino acid sequence of a 50 kDa putative hemin binding protein from P. intermedia was identical with Enolase from Streptococcus intermedia. Work is in progress to further characterize the molecular structure of these proteins.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosfopiruvato Hidratase
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Streptococcus
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Estrutura Molecular
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Proteínas de Transporte
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Sequência de Aminoácidos
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Porphyromonas
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Porphyromonas gingivalis
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Prevotella intermedia
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Prevotella
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Análise de Sequência de Proteína
Idioma:
Ko
Revista:
The Journal of the Korean Academy of Periodontology
Ano de publicação:
2006
Tipo de documento:
Article